Constituents | Percent |
Luria-Bertani bacterial growth media with bleach [NaCl + yeast extract + Tryptone] | 15% |
20mM tris-HCl [pH 7.5] + 1mM EDTA + 5% v/v Glycerol + 0.5mM [NH4]2SO4 + 0.1mM dithiothreitol + 1mM Phenylmethanesulfonyl fluoride[PMSF] in DMSO | 25% |
Water | 50% |
10mM Sodium Phosphate + 10mM NaCl + 0.2% Sodium Azide | 10% |
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